SPERM CRYO SYSTEM 0.25 ml

IVF CONSUMABLES

SCS-620

SPERM CRYO SYSTEM, 0.25 ml, Transparent,

For Sperm Cryopreservation

A. USAGE:

SPERM CRYO SYSTEM are specialized tool used for sperm cryopreservation in Assisted Reproductive Technologies (ART).

B. STORAGE:

C. STERILITY:

D. DESCRIPTION:

Straw length 133 mm, flexible, transparent, made of high-quality materials.
This straw is composed of two distinct sections separated by a white safety plug.

E. PRECAUTIONS:

Handling and storage:

This product is for laboratory use only and should be handled by trained professionals.
Always handle straws with cryo-safe gloves to avoid thermal injury.
Store straws in labeled canisters within the liquid nitrogen tank to prevent misidentification.

Avoid overfilling:

Overfilling straws can lead to cracking or rupture because the sample volume increase during freezing process that make excessive pressure.
To prevent these issues, always leave a small air gap inside the straw to accommodate expansion, and begin freezing in nitrogen vapor (-80 °C to -120 °C) for gradual cooling before immersing in liquid nitrogen (-196 °C).

Sterility:

Freezing process:

Always use a gradual cooling process to prevent thermal shock and ensure sample viability.

Labeling:

Use permanent, freezing resistant labels to prevent sample data under freezing conditions.

Equipment safety:

Use appropriate safety equipment when working with liquid nitrogen to avoid burns.

F. PROCEDURES:

Preparation:

Collect and evaluate the sample for quality, adjusting it with a cryoprotective freezing medium to protect sperm cells during freezing.
Verify that the straws are sterile and the package is intact before use.
Ensure all tools and cryoprotective agents are pre-prepared and at the required temperatures.
Maintain an aseptic environment to avoid contamination.

Filling & Sealing:

Fill straws with sample and seal them, ensuring proper labeling with details and freezing date.
Load the sample (e.g., semen sample) using a filling nozzle and a micro-aspirator or a syringe with a specific fitting.
Do not fill the straw completely, leave a small air gap inside the straw (about 10 % of the volume) to accommodate expansion during freezing.
The sample should rise through the white safety plug up to half of the powder.
Seal both ends of the straw securely using a heat sealer.
Label each straw clearly with sample ID, date, and any other necessary information.

Freezing:

Place straws horizontally in a controlled freezing device or in nitrogen vapor (-80 °C to -120 °C) for
15 – 30 minutes.
Transfer the straws into liquid nitrogen tank (-196 °C) for long term storage.

Thawing:

Retrieve the straw carefully from the liquid nitrogen using cryo-safe gloves and tools.
Place the straw in a water bath at the appropriate thawing temperature (e.g., 37 °C) for a few seconds.